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排序方式: 共有319条查询结果,搜索用时 312 毫秒
1.
Improved activity and thermostability of Candida antarctica lipase B by DNA family shuffling 总被引:2,自引:0,他引:2
Suen WC Zhang N Xiao L Madison V Zaks A 《Protein engineering, design & selection : PEDS》2004,17(2):133-140
DNA family shuffling was used to create chimeric lipase B proteins with improved activity toward the hydrolysis of diethyl 3-(3',4'-dichlorophenyl)glutarate (DDG). Three homologous lipases from Candida antarctica ATCC 32657, Hyphozyma sp. CBS 648.91 and Crytococcus tsukubaensis ATCC 24555 were cloned and shuffled to generate a diverse gene library. A high-throughput screening assay was developed and used successfully to identify chimeric lipase B proteins having a 20-fold higher activity toward DDG than lipase B from C.antarctica ATCC 32657 and a 13-fold higher activity than the most active parent derived from C.tsukubaensis ATCC 24555. In addition, the stability characteristics of several highly active chimeric proteins were also improved as a result of family shuffling. For example, the half-life at 45 degrees C and melting point (T(m)) of one chimera exceeded those of lipase B from C.antarctica ATCC 32657 by 11-fold and 6.4 degrees C, respectively, which closely approached the stability characteristics of the most thermostable parent derived from Hyphozyma sp. CBS 648.91. 相似文献
2.
Aleksandr Drozd Olaf Witkowski Satoshi Matsuoka Takashi Ikegami 《Artificial Life and Robotics》2016,21(3):317-323
We extend an abstract agent-based swarming model based on the evolution of neural network controllers, to explore further the emergence of swarming. Our model is grounded in the ecological situation, in which agents can access some information from the environment about the resource location, but through a noisy channel. Swarming critically improves the efficiency of group foraging, by allowing agents to reach resource areas much more easily by correcting individual mistakes in group dynamics. As high levels of noise may make the emergence of collective behavior depend on a critical mass of agents, it is crucial to reach sufficient computing power to allow for the evolution of the whole set of dynamics in simulation. Since simulating neural controllers and information exchanges between agents are computationally intensive, to scale up simulations to model critical masses of individuals, the implementation requires careful optimization. We apply techniques from astrophysics known as treecodes to compute the signal propagation, and efficiently parallelize for multi-core architectures. Our results open up future research on signal-based emergent collective behavior as a valid collective strategy for uninformed search over a domain space. 相似文献
3.
Abstract. A proper coloring of the edges of a graph G is called acyclic if there is no two-colored cycle in G . The acyclic edge chromatic number of G , denoted by a'(G) , is the least number of colors in an acyclic edge coloring of G . For certain graphs G , a'(G)\geq Δ(G)+2 where Δ(G) is the maximum degree in G . It is known that a'(G)≤ Δ + 2 for almost all Δ -regular graphs, including all Δ -regular graphs whose girth is at least cΔ log Δ . We prove that determining the acyclic edge chromatic number of an arbitrary graph is an NP-complete problem. For graphs
G with sufficiently large girth in terms of Δ(G) , we present deterministic polynomial-time algorithms that color the edges of G acyclically using at most Δ(G)+2 colors. 相似文献
4.
Kseniia A. Sergeeva Dmitrii V. Pavlov Albert A. Seredin Eugeny V. Mitsai Aleksandr A. Sergeev Evgeny B. Modin Anastasiia V. Sokolova Tsz Chun Lau Kseniia V. Baryshnikova Mihail I. Petrov Stephen V. Kershaw Aleksandr A. Kuchmizhak Kam Sing Wong Andrey L. Rogach 《Advanced functional materials》2023,33(44):2307660
In order to advance the development of quantum emitter-based devices, it is essential to enhance light-matter interactions through coupling between semiconductor quantum dots with high quality factor resonators. Here, efficient tuning of the emission properties of HgTe quantum dots in the infrared spectral region is demonstrated by coupling them to a plasmonic metasurface that supports bound states in the continuum. The plasmonic metasurface, composed of an array of gold nanobumps, is fabricated using single-step direct laser printing, opening up new opportunities for creating exclusive 3D plasmonic nanostructures and advanced photonic devices in the infrared region. A 12-fold enhancement of the photoluminescence in the 900–1700 nm range is observed under optimal coupling conditions. By tuning the geometry of the plasmonic arrays, controllable shaping of the emission spectra is achieved, selectively enhancing specific wavelength ranges across the emission spectrum. The observed enhancement and shaping of the emission are attributed to the Purcell effect, as corroborated by systematic measurements of radiative lifetimes and optical simulations based on the numerical solution of Maxwell's equations. Moreover, coupling of the HgTe photoluminescence to high quality factor modes of the metasurface improves emission directivity, concentrating output within an ≈20° angle. 相似文献
5.
Microwave Sol–Gel Synthesis of CaGd2(MoO4)4:Er3+/Yb3+ Phosphors and Their Upconversion Photoluminescence Properties 下载免费PDF全文
Chang Sung Lim Victor Atuchin Aleksandr Aleksandrovsky Maxim Molokeev Aleksandr Oreshonkov 《Journal of the American Ceramic Society》2015,98(10):3223-3230
CaGd2(MoO4)4:Er3+/Yb3+ phosphors with the doping concentrations of Er3+ and Yb3+ (x = Er3+ + Yb3+, Er3+ = 0.05, 0.1, 0.2, and Yb3+ = 0.2, 0.45) have been successfully synthesized by the microwave sol–gel method, and the crystal structure refinement and upconversion photoluminescence properties have been investigated. The synthesized particles, being formed after heat‐treatment at 900°C for 16 h, showed a well‐crystallized morphology. Under the excitation at 980 nm, CaGd2(MoO4)4:Er3+/Yb3+ particles exhibited strong 525 and 550‐nm emission bands in the green region and a weak 655‐nm emission band in the red region. The Raman spectrum of undoped CaGd2(MoO4)4 revealed about 15 narrow lines. The strongest band observed at 903 cm?1 was assigned to the ν1 symmetric stretching vibration of MoO4 tetrahedrons. The spectra of the samples doped with Er and Yb obtained under 514.5 nm excitation were dominated by Er3+ luminescence preventing the recording Raman spectra of these samples. Concentration quenching of the erbium luminescence at 2H11/2→4I15/2 and 4S3/2→4I15/2 transitions in the CaGd2(MoO4)4:Er3+/Yb3+ crystal structure was established to be approximately at the 10 at.% doping level. 相似文献
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7.
Impett Emily A.; Gordon Amie M.; Kogan Aleksandr; Oveis Christopher; Gable Shelly L.; Keltner Dacher 《Canadian Metallurgical Quarterly》2010,99(6):948
In 2 daily experience studies and a laboratory study, the authors test predictions from approach-avoidance motivational theory to understand how dating couples can maintain feelings of relationship satisfaction in their daily lives and over the course of time. Approach goals were associated with increased relationship satisfaction on a daily basis and over time, particularly when both partners were high in approach goals. Avoidance goals were associated with decreases in relationship satisfaction over time, and people were particularly dissatisfied when they were involved with a partner with high avoidance goals. People high in approach goals and their partners were rated as relatively more satisfied and responsive to a partner's needs by outside observers in the lab, whereas people with high avoidance goals and their partners were rated as less satisfied and responsive. Positive emotions mediated the link between approach goals and daily satisfaction in both studies, and responsiveness to the partner's needs was an additional behavioral mechanism in Study 2. Implications of these findings for approach-avoidance motivational theory and for the maintenance of satisfying relationships over time are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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9.
Sergey G. Kopysov Aleksandr A. Erofeev 《The International journal of environmental studies》2015,72(3):380-385
The landscape conditions for forming the water balance and runoff from catchment areas can change considerably under the pressure of natural and anthropogenic factors. The role of hydromorphic transformation of landscapes is important for most of the Ob basin; it results in the paludification of the area. The specific parameter n is used in calculating water balance according to Mezentsev’s method of hydro-climatic calculations to take account of landscape conditions. We suggest determining this parameter’s value by geomorphological features of the landscapes using GIS tools. 相似文献
10.
Amir Pnueli Aleksandr Zaks Lenore Zuck 《Electronic Notes in Theoretical Computer Science》2006,144(4):73
We consider the problem of a module interacting with an external interface (environment) where the interaction is expected to satisfy some system specification Φ. While we have the full implementation details of the module, we are only given a partial external specification for the interface. The interface specification being partial (incomplete) means that the interface displays only a strict subset of the behaviors allowed by the interface specification.Based on the assumption that interface specifications are typically incomplete, we address the question of whether we can tighten the interface specification into a strategy, consistent with the given partial specification, that will guarantee that all possible interactions resulting from possible behaviors of the module will satisfy the system specification Φ. We refer to such a tighter specification as Φ-guaranteeing specification. Rather than verifying whether the interface, which is often an off-the-shelf component, satisfies the tighter specification, the paper proposes a construction of a run-time monitor which continuously checks the existence of a Φ-guaranteeing interface.We view the module and the external interface as players in a 2-player game. The interface has a winning strategy if it can guarantee that no matter what the module does, the overall specification Φ is met. The problem of incomplete specifications is resolved by allowing the interface to follow any strategy consistent with the interface specification. Our approach essentially combines traditional run-time monitoring and static analysis. This allows going beyond the focus of traditional run-time monitoring tools – error detection in the execution trace, towards the focus of the static analysis – bug detection in the programs. 相似文献